RAA Basic Nucleic Acid Amplification Kit (RUO)
Rapid amplification of DNA at 37 ℃; product confirmed by agarose gel electrophoresis. Runs on a heat block, water bath or incubator.
Cat. No. S001ZCRequest a Quote
RAA · Recombinase-Aided Amplification
Isothermal nucleic acid detection. Results in 5–15 minutes.
RAA runs at a constant 37–42 ℃ with no thermal cycler. Lyophilised reagents and a portable fluorescence analyzer bring molecular-level testing to the bench, the plant floor and the field.
37–42 ℃Reaction temperature
5–15 minTime to result
1 %LOD · meat adulteration
10³ cfu/mLLOD · foodborne pathogens
29 SKUsReagents & instruments (RUO)
01 · Technology
RAA replaces thermal cycling with enzymes.
Recombinase-Aided Amplification is the isothermal amplification platform behind every ZC Bioscience reagent. All kits are developed and manufactured in-house.
How it works
A recombinase obtained from bacteria or fungi binds tightly to the primer DNA at ambient temperature, forming a recombinase–primer complex. When the primer locates its fully complementary sequence on the template, single-stranded DNA-binding protein helps open the double strand, and DNA polymerase synthesises a new complementary strand. The product accumulates exponentially.
With a labelled fluorescent probe the reaction can be read in real time; a fluorescence result is typically available within 5–15 minutes. Reagents are pre-mixed and lyophilised in the tube: add Buffer A, Buffer B and the extracted sample nucleic acid, then read on the Genchek analyzer or a standard real-time instrument.
STEP 01
Recombinase binds primer
Recombinase–primer complex forms at ambient temperature.
STEP 02
Target search & strand opening
Complex scans the template; SSB stabilises the opened strands.
STEP 03
Polymerase extension
Strand-displacing DNA polymerase extends from the primer 3' end.
STEP 04
Exponential readout
Probe cleavage gives a real-time fluorescence curve in 5–15 min.
Simple operation
Molecular-level testing that does not require specialist staff. A two-step workflow: extract, then amplify and read.
Fast
Results in 5–15 minutes with a fluorescent probe; gel-readable product within 30 minutes for basic kits.
Accurate
High sensitivity and specificity — the recombinase–primer complex recognises the target sequence by base pairing.
Portable
Compact instruments suited to harsh field conditions and on-site inspection — the Genchek analyzer weighs 3.8 kg.
Isothermal
Runs at 30–42 ℃ (optimum 37–39 ℃). No thermal cycler or other expensive equipment required.
Lyophilised
Reagents supplied as freeze-dried pellets: ready to use, easy to transport, and free from freeze–thaw loss.
platform-comparison · RAA vs PCR vs LAMP
| Parameter | RAA | PCR | LAMP |
|---|---|---|---|
| Enzymes | Recombinase, SSB, strand-displacing DNA polymerase | Taq DNA polymerase | Strand-displacing DNA polymerase |
| Temperature | 37–39 ℃, constant | High, cycling | 60–65 ℃ |
| Time | 15 min | 1.5 h | 40 min |
| Primer pairs | 1 | 1 | 2 |
| Format | Lyophilised pellet | Liquid | Liquid |
| Readout | Gel · real-time fluorescence · lateral-flow strip | Gel · real-time fluorescence | Visual turbidity |
| Limitation | — | Slow; thermal cycler required | Aerosol carry-over; false positives |
Typical values for ZC Bioscience RAA kits versus conventional workflows. All reagents are for research use only.
02 · Products
Five product lines, one isothermal platform.
RAA core amplification kits, rapid extraction, the Genchek fluorescence analyzer, 17 food-safety detection kits and a fully automated system. Every reagent is supplied lyophilised and for research use only (RUO).
Molecular detection enzyme kits for DNA and RNA targets. 48 tests per kit, 50 μL reaction, lyophilised; store at -20 ℃, 12-month shelf life. Primers 30–35 bp, probes 46–52 bp. Over 10⁷-fold amplification of the target.
Request the catalogueRAA Fluorescent Nucleic Acid Amplification Kit (RUO)
Real-time monitoring on the Genchek analyzer or standard real-time instruments (ABI, MJ, Roche, Bioer series); 5–20 min.
Cat. No. S002ZCRequest a Quote
RT-RAA Basic Nucleic Acid Amplification Kit (RUO)
RNA is first reverse-transcribed to cDNA, then amplified at 37 ℃; gel readout. Heat block, water bath or incubator.
Cat. No. S003ZCRequest a Quote
RT-RAA Fluorescent Nucleic Acid Amplification Kit (RUO)
Rapid amplification of RNA with real-time fluorescence monitoring on Genchek or standard real-time instruments; 5–20 min.
Cat. No. S004ZCRequest a Quote
RAA-nfo Nucleic Acid Amplification Kit, Lateral-Flow (RUO)
39 ℃ isothermal amplification; nfo endonuclease cleaves the probe to yield a dual-labelled amplicon read on a universal nucleic acid strip.
Cat. No. S005ZCRequest a Quote
RT-RAA-nfo Nucleic Acid Amplification Kit, Lateral-Flow (RUO)
42 ℃ isothermal amplification of RNA targets with reverse transcription; result read on a universal lateral-flow strip.
Cat. No. S006ZCRequest a Quote
Genchek Isothermal Fluorescence Analyzer
An integrated, portable analyzer for on-site and mobile testing. Built-in reagent mixing, fully matched to RAA reagents; dual fluorescence channels; all assays run under one software interface with automatic data analysis and report generation. 8 wells × 2 channels — up to 16 targets per run.
- Throughput8 wells
- ChannelsFAM / ROX
- Temperature rangeAmbient to 65 ℃
- Temperature accuracy±0.5 ℃
- Net weight3.8 kg
- Dimensions (L×W×H)255 × 240 × 123 mm
- Power120 W · AC 100–240 V, 50/60 Hz
- Operating environment5–35 ℃ · 20–80 % RH
25+ compatible RAA assaysRequest a Quote
Purpose-built to yield RAA-ready DNA or RNA. No hazardous solvents and low cross-contamination risk. Store at room temperature, dry; 12-month shelf life.
Request the catalogue
Rapid DNA Extraction Reagent (RUO)
Genomic DNA from animal tissue, blood, meat products, plant tissue, seeds and bacteria. No organic extraction, no ethanol precipitation.
Cat. No. ND01ZCRequest a Quote
Universal Rapid DNA Extraction Kit, Spin Column (RUO)
Lyse–bind–wash–elute in 15 minutes. Animal tissue, cells, bacteria, saliva, blood, swabs, faeces, bone and feed.
Cat. No. ND02ZCRequest a Quote
Universal Rapid RNA Extraction Kit, Spin Column (RUO)
Four steps, 10 minutes, high-quality RNA from animal tissue, cells, saliva, blood, swabs and faeces.
Cat. No. ND03ZCRequest a Quote
Magnetic-Bead DNA Extraction Pre-filled Plate (RUO)
For automated magnetic-bead extractors. High-throughput, consistent yield; cuts hands-on time for food-safety, environmental and molecular-biology research.
Cat. No. ND06ZCRequest a Quote
17 RAA fluorescent detection kits (RUO), 24 tests per kit, lyophilised; store at -20 ℃, 6-month shelf life. Compatible with Genchek and standard real-time instruments (ABI, MJ, Roche, Bioer series). Result read directly from the amplification curve — no electrophoresis.
Request the catalogue
Animal-Derived Ingredient Molecular Detection Kits (RAA, Fluorescent) (RUO)
Species-specific gene fragments at 30–42 ℃; no cross-reaction with common species; detects different breeds. For animal tissue, blood, foods, meat products (incl. processed meat) and feed.
- Chicken
- Duck
- Pork
- Bovine
- Sheep & goat
- Rat
- Horse
- Mink
- Fox
- Donkey
Cat. No. A101ZC–A110ZCRequest a Quote
Foodborne Pathogen Molecular Detection Kits (RAA, Fluorescent) (RUO)
Qualitative detection of a specific gene fragment in 5–15 minutes. For foods, beverages, water, cosmetics, disposable hygiene products and seafood. Method reference: GB 4789 series, SN/T series.
- Staphylococcus aureus
- Salmonella
- Pseudomonas aeruginosa
- Listeria monocytogenes
- Haemolytic Streptococcus
- Vibrio parahaemolyticus
- Shigella
Cat. No. B201ZC–B207ZCRequest a Quote
GMO Ingredient Molecular Detection Kits (RAA, Fluorescent) (RUO)
Specific detection of exogenous elements and events in crops and agricultural products, 24 tests per kit. Element screening plus event-specific kits for soybean GTS 40-3-2 and MON89788, maize MON810 and Bt11, canola T45 and Oxy-235.
- Bt
- bar
- hpt
- CaMV 35S promoter
- FMV 35S promoter
- nos terminator
Cat. No. on requestRequest a Quote
Genchek Fully Automated Isothermal Nucleic Acid Detection System
Sample in, result out in about 30 minutes. One instrument performs pipetting, lysis, nucleic acid purification, isothermal amplification, fluorescence detection, analysis and reporting — load the sample and the system does the rest.
- Run time~30 min
- Sample throughput1 per run
- ChannelsFAM / ROX
- Interface7-inch colour touchscreen
- UV lamp · air purificationYes · Yes
- Dimensions (L×W×H)260 × 325 × 420 mm
- Power221 W · AC 220 V, 50 Hz
- Pipetting (15–50 μL)Accuracy >98.5 %, CV <3 %
Product photography on requestRequest a Quote
03 · Solutions
Three food-safety workflows, one 15-minute run.
Isothermal RAA reagents recognise and amplify the target gene fragment; the Genchek analyzer monitors amplification in real time and the run completes in 15 minutes.
Meat species authentication
Animal-Derived Ingredient Detection
As price gaps between meats widen, species substitution and adulteration are increasingly common. RAA species kits detect species-specific genes, identifying meat origin at the molecular level with no cross-reaction between species.
10species targets
1 %adulteration LOD
- Chicken
- Pork
- Duck
- Beef
- Sheep/goat
- Rat
- Horse
- Mink
- Fox
- Donkey
Users: food and drug regulators, customs and border inspection, livestock authorities, agricultural research institutes, law enforcement; food, dietary-supplement and feed manufacturers; third-party laboratories.
Foodborne pathogens
Foodborne Pathogen Detection
Pathogenic bacteria account for an estimated 40–50 % of reported foodborne-illness cases in China each year. RAA pathogen kits combine multi-channel detection, short run times and high sensitivity — raising throughput and moving food-safety warnings earlier.
9pathogen targets
10³cfu/mL LOD
- S. aureus
- Salmonella
- P. aeruginosa
- L. monocytogenes
- Haemolytic Streptococcus
- V. parahaemolyticus
- Shigella
- E. coli O157:H7
- V. cholerae
Users: food and drug regulators, customs and border inspection, public-health laboratories; food and cosmetics manufacturers; third-party laboratories. Method reference: GB 4789 series, SN/T series.
GMO screening
Transgenic Ingredient Detection
GM crops carry genes introduced to alter traits, nutrition or quality, and regulators worldwide require labelling and control. RAA GMO kits specifically detect exogenous elements and events in crops, identifying GM material at the molecular level.
6screening elements
6event-specific kits
- Bt
- bar
- hpt
- CaMV 35S
- FMV 35S
- nos
Users: food and drug regulators, customs and border inspection, livestock authorities, agricultural research institutes; food, dietary-supplement and feed manufacturers; third-party laboratories.
04 · Services
Support from first run to custom target.
Our R&D team answers every product question with patient explanation and demonstration — and develops new RAA assays when your target is not yet in the catalogue.
Technical support
Consultation, technical documentation, training and demonstration. Example workflow for a species assay: 50 mg sample into extraction solution, 70 ℃ for 10 min; add 45.5 μL Buffer A, 2.5 μL Buffer B and 2 μL sample nucleic acid; read on Genchek in 5–15 min.
After-sales service
Long-term technical documentation and support. If a problem arises, contact +86 571 8861 9668 or zcsw2017@163.com — our engineers respond promptly and resolve issues remotely by email, online or by phone. Mon–Fri 9:00–18:00 (UTC+8).
Custom assay development
RAA enables portable, rapid nucleic acid testing across pathogen detection, meat adulteration, GMO screening and more. We develop custom RAA assays for new targets and offer OEM supply — contact us to discuss your target.
05 · Company
R&D-driven, Hangzhou-based.
Hangzhou ZC Bio-Sci & Tech Co., Ltd. ("ZC Bioscience") is an R&D-driven biotechnology company based in Hangzhou, China. We develop and apply Recombinase-Aided Amplification (RAA), an isothermal nucleic acid amplification technology, and supply RAA-based reagents, extraction kits and portable fluorescence instruments for rapid molecular testing.
Since September 2016 the company has accelerated product development for RAA-based rapid food-safety testing, launching in turn the animal-species, foodborne-pathogen and GMO molecular detection series. RAA is also applied to environmental monitoring, aquaculture pathogen detection and animal disease detection. We have signed strategic cooperation agreements with government institutions and research organisations across several fields to co-develop RAA-based detection kits.
500 m²Laboratory space, plus 400 m² of office space
RMB 12M+Installed equipment value
90 %+R&D team holding master's degrees or higher
5050Hangzhou Binjiang District "5050 Talent Program"; key enterprise cultivated by the district
06 · News
Conferences, explainers and literature.
Company news from the food-safety testing community.
Literature | Rapid Nucleic Acid Amplification by Recombinase-Aided Amplification
In vitro nucleic acid amplification enables efficient, rapid amplification of trace nucleic acids and has been widely applied in molecular biology and forensic identification. Recombinase-aided amplification is an isothermal in vitro method developed on established amplification principles.
Explainer | Recombinase-Aided Rapid Nucleic Acid Amplification
Recombinase-aided amplification (RAA) is an isothermal in vitro amplification technology in which recombinase, single-stranded DNA-binding protein and DNA polymerase replace the thermal-cycling denaturation of conventional PCR, allowing rapid amplification at a constant 37 ℃.
Highlights | RAA Technology Debuts at the Food Microbiology Detection & Control Exchange
On 20–22 July 2017 the 7th Food Microbiology Detection and Control Technology Exchange was held in Shanghai, jointly organised by the Agro-product Safety Research Center of the Chinese Academy of Inspection and Quarantine, the Analytical Microbiology Committee of the Chinese Society for Microbiology, and Foodmate.
Forum | 7th Food Microbiology Detection & Control Technology Exchange (19–22 July)
Foodborne disease caused by pathogenic microorganisms is the number-one food-safety issue. Controlling contamination, strengthening laboratory capability and applying advanced microbial detection and control technology in production have become focal concerns for regulators and food companies alike.
ZC Bioscience at the 10th China International Food Safety & Quality Conference (CBIFS 2017)
The annual CBIFS food-safety technology forum was held on 7–8 April 2017 at the Hangzhou International Expo Center. ZC Bioscience, a newcomer to food-safety testing, made its debut at the conference.
Get started
Bring your target to an isothermal workflow.
Tell us your sample type and target. We will recommend a kit, quote pricing and lead time, and discuss custom development if your target is not yet in the catalogue.
07 · Contact
Talk to the team.
Distribution enquiries, quotations and technical questions are welcome. We reply within one business day.
- Company
- Hangzhou ZC Bio-Sci&Tech Co. Ltd
- Address
- Tianhe Hi-Tech Park, Bldg 5, 688 Bin'an Rd, Binjiang District, Hangzhou 310052, China
- zcsw2017@163.com
- Phone
- +86 571 8861 9668
- Hours
- Mon–Fri 9:00–18:00 (UTC+8)